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yvcK [2017-05-31 13:59:14]
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yvcK [2017-05-31 13:59:14]

essential for growth under gluconeogenetic conditions, required for correct localization of PBP1
locus
BSU34760
pI
5.41
mw
34.53 kDa
protein length
317 aa Sequence Blast
gene length
951 bp Sequence Blast
function
localization of ponA|PBP1
product
unknown
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.1|Biosynthesis of peptidoglycan]
  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.2|Cell shape]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.1|Biosynthesis of cell wall components] → [category|SW 2.6.1.1|Biosynthesis of peptidoglycan]
  • [category|SW 6|Groups of genes] → [category|SW 6.11|Efp-dependent proteins]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.6|Phosphorylation on a Thr residue]

    • Gene

    Coordinates
    3,570,546 → 3,571,499

    Phenotypes of a mutant

  • unable to grow with gluconeogenic substrates as single carbon source [Pubmed|16272399]
  • filamentous or L-shape-like aberrant morphologies (suppressed by Mg ) [Pubmed|16272399], this is supressed by overexpression of [protein|A4C8719E06F774A6EB4D79757CC79CF89E453A54|MreB] or by deletion of ''[gene|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|ponA]'' ([gene|A8EEC896B279CFDDE9C3A6F146A04EC822E33A4F|PBP1]]] ) [Pubmed|21320184]

    • The protein

    Protein family

  • UPF0052 family (according to Swiss-Prot)

    • [SW|Domains]

  • phosphorylated on Thr-304 by [protein|23FF4A00C36EC1B7297F51A9EF3579B41F0B7EBA|PrkC], dephosphorylated by [protein|84447F9A6644EA8A7593BB99B2B69D4377E670E2|PrpC] [Pubmed|25012659]

    • Structure

  • [PDB|2O2Z] (the protein from ''B. halodurans'', 63% identity, 86% similarity)

    • [SW|Localization]

  • localized as a helical-like pattern [Pubmed|25012659,21320184]

    • Expression and Regulation

    Operons

    genes
    [gene|0C40455EB53D25363FC3EB0E84502A76520F5F91|nahA]-[gene|EB12C6B4F36B57BF0C59CE048CBAE18734C78D35|yvcJ]-[gene|7150BABA5086D5E2EB8102E4901A216F43576282|glmR]-[gene|1986DB3078FBA16D6ACCD70EEAC95C46564D0958|whiA]-[gene|A269774F2FDC94F93BA5F1360FFFE754B50383AD|crh]-[gene|C521BEE1EEEA7AD283A3C47C34301A8603CD9188|yvcN]
    description
    [Pubmed|9237995]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|16272399], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

    • regulation

  • constitutive expression at both protein and RNA levels [Pubmed|24097947,22383849]

    • additional information

  • A [protein|search|ncRNA] is predicted between '[protein|search|yvcI]' and '[protein|search|trxB]' [PubMed|20525796]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-B640 (yvcK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1639 NBRP B. subtilis, Japan]

    • Expression vector

  • pGP736 (N-terminal Strep-tag, purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Stülke] lab

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW| Görke] lab

    • Labs working on this gene/protein

  • [SW|Anne Galinier], University of Marseille, France

    • References

    Reviews

  • 21371139

    • Original publications

  • 9237995,16272399,21320184,25012659,25047842,27806131